Genetic adaptations help Amazonian populations resist Chagas infection

The research has analysed the genomic data of 118 contemporary individuals from 19 different populations of the Amazon to find traces of genetic adaptation to the Amazon rainforest environment.

“We focused on finding evidence of positive natural selection related to tropical diseases in the Americas.” Points out Tábita Hünemeier, Principal Investigator at the Institute of Evolutionary Biology (IBE-CSIC-UPF) who led the study.

Through the analysis of the genomes, the study found a high-frequency variant of the PPP3CA gene in the inhabitants of Amazonia that could be responsible for this resistance. To verify its effectiveness, functional studies were carried out in the laboratory. In this way, the study discovered that the variant of the PPP3CA gene reduces the risk of infection by the Chagas pathogen.

Functional studies on the immunological action of the PPP3CA gene against the Chagas parasite

The PPP3CA gene codes for a key protein in the activation of immune cells, the innate immune response, and the internalization of the T.cruzi parasite in human cells. This study detected a variant of this gene in a very abundant way in Amazonian populations that is expressed in heart tissue and in immune cells.

The study performed functional studies with heart cell tissue using stem cells, some of which contained the PPP3CA gene variant found in high frequency in Amazonian populations. The results showed that there is less internalization of the parasite in heart cells when they have the gene variant.

“The presence of the PPP3CA gene variant could be the cause of milder disease or less infection in these populations.” Adds David Comas, Professor of Biology in the Department of Medicine and Life Sciences (MELIS) at the UPF, Principal Investigator at IBE (CSIC-Pompeu Fabra University) and co-author of the research.

Positive natural selection in the Amazonian population due to a pathogen

Study results estimate that the natural selection for Chagas disease resistance began 7,500 years ago after the Amazonian population separated from the Andean and Pacific coast populations.

Supported by previous studies with 9,000-year-old samples, the research concludes that the epidemics would have positively selected the individuals with the greatest resistance to tropical diseases, such as Chagas disease, generating a unique resistance in this population.

Behavioral genetic adaptations to life in the jungle

Additionally, the study found genetic adaptations associated with behavioral traits such as “Novelty-seeking behaviors,” a genetic trait that determines the search for new experiences. According to the study, this trait could have been crucial to the hunter-gatherer lifestyle of Amazonian populations in the past, as it enabled them to explore new territories and search for resources.

Cardiovascular and metabolic traits

The study also detected cardiovascular and metabolic traits that are consistent with the genetic predominance observed in previous research, since a 66% rate of obesity, diabetes, and coronary heart disease had already been detected in some Amazonian populations.